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Prueba Grados

Publicado el: 25/2/2021
The CCT collected in both experiments (n = 20 poults/group) were homogenized and diluted with saline (1:4 w/v). CCT homogenate samples were diluted by ten-fold serial dilutions and 100 μL were plated on brilliant green agar (BGA, Catalog no. 70134, Sigma St. Louis, MO, USA) plates containing 25 μg/mL novobiocin (NO, catalog no. N-1628, Sigma St. Louis, MO, USA) and 20 μg/mL of nalidixic acid (NA, catalog no. N-4382, Sigma, St. Louis, MO, USA), incubated at 37 ?C for 24 h, then enumerated for total Salmonella enterica serovar Enteritidis cfu. Following plating to enumerate total Salmonella enterica serovar Enteritidis, the CCT homogenate samples were enriched with tetrathionate enrichment broth, 1× final dilution, and further incubated at 37 ?C for 24 h. Enrichment samples were streaked onto XLT-4) selective media for confirmation of Salmonella presence. Plates that were negative on the enumeration method but were positive on enrichment were considered as 500 cfu/g as the limit of detection for SE viability.