Shenzhen Lvshiyuan Biotechnology Co. Ltd. (China)
Shenzhen Lvshiyuan Biotechnology Co. Ltd. is a high-tech enterprise focused on developing the most advanced animal disease diagnostic kit, food safety test kit, environmental monitoring reagent, new enzyme reagent, microorganism reagent, protein product, nucleotide product, green aquatic drugs product, small molecular antigen, antibody, biochemistry product, etc.

Commercial Reaching Zone: North America, Central America, South America, Europe, Oceania, Africa, Asia, China

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PRRSV RT-PCR test kit

Usage
This test was assessed for sensitivity and application as a rapid diagnostic tool by comparison with virus isolation and detection of PRRS virus antibody in blood. The RT-PCR test was slightly more sensitive than virus isolation for detection of virus in serum and markedly more sensitive than virus isolation from plasma from experimentally infected pigs.

Principle
1 RNA in refrigerated serum sample is extracted by TRIzol , The cDNA synthesis was performed with RNA template and specific primer . To produce fragment of PRRSV , degenerate at high temperature, anneal at low temperature, transcript at mid-temperature, cycle with Taq DNA , amplify million fold through 35 times cycle. Electrophoresize RT-PCR products , dyed by ETBR, generate specific fragments in the ultraviolet lights which can be seen by eyes.

2 Components:
A solution: Positive control 60 ul
B solution: TriZol solution 7ml
C solution: CHCl3 3.5ml
D solution: C3H3O 4ml
E solution: CH3CH2OH 12ml
F solution: DEPC-Deionized water: 500ul
G solution:RT-PCR buffer 22.5ul X11
H solution:sample buffer: 50ul

Storage: B, C,D,H, stored at 4-8 ¡æ A,E,F,G, stored at -20 ¡ædon¡¯t froze repeatedly.

Expiry date:3 months.

Specification:10 test

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ENGOREPROD 828-2432 20080724

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