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Low-Matrix Mycotoxin Kits
Aflatoxins and ochratoxin A in commodities which pose difficult problems in measurement by conventional “rapid” ELISA methods.
The so-called rapid ELISA method provides for a quicker and simpler assay with less manipulation by the operator and is used where speed and simplicity are at a premium, for instance when large truckloads of grain must be screened for mycotoxin content
The main drawback with this type of assay is the well known ‘matrix effect’. These problems are compounded by the fact that a mycotoxin assay may be used with many different kinds of commodity extract, and controlling for matrix effect in one commodity by, for instance, using an antigen-free extract in the assay would only correct for the effects of that particular matrix and would likely be incorrect for others. A case in point is the assay of aflatoxin in silage, a vegetable matter usually a byproduct of grain harvests preserved by fermentation in a large enclosed structure or silo and is an important component of cattle fodder. Immunoassay of aflatoxin in silage extract in a conventional rapid assay leads to high false positive values in samples that contain no aflatoxin by the HPLC gold standard.
A similar situation exists in the case of ochratoxin A. While most grains and cereals, with a relatively moderate matrix effect, may be screened for ochratoxin content using the simultaneous incubation of sample and conjugate, commodities such as coffee, cocoa, spices, and grape derivatives such as red wine are notoriously difficult to test due to “matrix effect”.
As a solution to this problem, Helica Biosystems, Inc has successfully devised immunoassays to measure total aflatoxins and ochratoxin in silage, coffee and other high matrix effect commodities at biologically useful levels. These low matrix assays are now available for distribution world-wide (see test list in Appendix). We are pleased to present the following set of data from a study we conducted to validate the field utility of this method.
It is difficult to determine at this point whether the low (<2ppb) values measured in some silage samples represents residual matrix effect or the cumulative measurement of several aflatoxin types (B1,B2,G1,G2) present at less than 1 ppb levels. Otherwise the Helica Biosystems low matrix ELISA is a cost effective screening tool for total aflatoxin content in a wide variety of commodities destined for animal or human consumption.
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