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Evaluation of recombinant Omp31 subcellular vaccine against Brucella ovis in rams |
 
Who saw this article? New!
Author: Silvia M. Esteina, Pablo C. Cheves, María A. Fiorentino, Juliana Cassataro, Fernando A. Paolicchi, Raúl A. Bowden (INTA)
Publication date: 05/18/2007
Brucella ovis causes an infectious disease in sheep characterized by epididymitis and infertility in rams and abortion in ewes. Vaccination programs are the only viable means for the control of B. ovis in countries with a high incidence. B. melitensis Rev.1, is considered the best vaccine for the prophylaxis of ovine brucellosis but has important disadvantages.
Accordingly, research is under way to develop subcellular vaccines. Detergent-extracted recombinant Omp31 (rOmp31 extract) from B. melitensis was previously identified as a protective immunogen against B. ovis in mice.
Objective
To evaluate the immunogenicity and protective activity of rOmp31 extract against B. ovis in rams.
Materials and methods
|
Group |
Rams per groupa |
Immunogenb |
Doses/ram/immunization |
|
|
1st &2nd |
3rd |
|
G1 |
4 |
rOmp31extract |
50 µg |
500 µg |
|
G2 |
4 |
E. coli (pUCI9) control |
50 µg |
500 µg |
|
G3 |
3 |
(PBS) (control) |
- |
- |
|
aRomney Marsh rams, bImmunogens were emulsified with Marcol 52 (kindly provided by Bayer, Argentina) |
Experimental design
 |
| Obtention of rOmp31 extract |
 |
Blood samples were taken at different times to evaluate antibody response in serum by ELISA
a) testes, epididymes, seminal vesicles, ampullaes and prostatic glands were subjected to bacteriological and histopathological examination |
Results
Discussion and conclusion
Immunization with Omp31 from B. melitensis induced humoral and cellular mechanisms in sheep and reduced histopatological lesions in immunized rams. |
References
- Blasco J.M. 1990. Brucella ovis, In: Nielsen K. Duncan J.R., editors. Animal Brucellosis
- Bowden R.A, et al., 1995. J. Med. Microbiol. 43, 344-347.
- Estein S.M. et al., 2003. Microbes Infect. 5, 85-93.
- Estein S.M. et al., 2004. Vet. Microbiol. Article in press.
Authors: Silvia M. Esteina*, Pablo C. Chevesb, María A. Fiorentinob, Juliana Cassataroc, Fernando A. Paolicchib, Raúl A. Bowdena
a) Laboratorio de Inmunología, Facultad de Ciencias Veterinarias, UNCPBA, (7000) Tandil, Buenos Aires, Argentina.
* CIC, Bs. As., Argentina.
b) Laboratorio de Bacteriología, Area de Producción Animal, INTA-Balcarce.
CONICET, Bs. As. Argentina.
c) Laboratorio de Inmunogenética, Hospital de Clínicas "José de San Martín", Facultad de Medicina. UBA, Bs. As., Argentina.
Author: Silvia M. Esteina, Pablo C. Cheves, María A. Fiorentino, Juliana Cassataro, Fernando A. Paolicchi, Raúl A. Bowden (INTA)
Publication date: 05/18/2007
Who saw this article? New!
 DISCUSSIONS ON THIS ISSUE.
 
| 06/06/2007 |
 |
|
ibrahim ibrahim
Profesor Doctor Microbiology/animal Reproductive Reserch Institute
Al Qahirah - Egypt |
|
Brucella melitensis is the main cause of brucellosis in the Middle East.
If this research done on brucella melitensis in project with my institute,
we can sell this vaccine in the Middle East
and we solve the problem of brucella melitensis infection in cows and camels beside sheep.
Your friend,
Ibrahim
Prof.Dr and Head of Reproductive Desease Departement
Animal Reproductive Research Institute
Egypt Giza ElHaram P.O.B.12556 Al-hram | Answer Checked by Engormix.com  |
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