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Phytex 500
TECHNICAL SUMMARY
What is PHYTEX 500
Phytase is an enzyme found in nature mainly in plants . It is formed in the stage
of sprouting to supply phosphorous during plant growth. Phytase is also found
in the small intestine mucous of many animals, and in case of micro-organisms,
it is found in Aspergillus sp. and yeast. Its is obtained by a NON-GMO natural
process from Aspergillus Níger.
Mode of action
Inositol Hexaphosphate , (IH), present in grains and beans, contain one to three
percent of (IH) , of which, 60% to 80% is phosphoric acid. Wheat:0.6 to 1.4
% (w/w); Bran:2.6 to 5.4 % (w/w) ; Soy Flakes:1.5 to 1.8% (w/w) Prot. Concr:
2.0 to 3.0% (w/w) Phytase breaks down the (IH) molecule releasing its phosphoric
acid content making it bioavailable to the animal, thus reducing the need for
inorganic phosphate.
PHYTEX 500 Savings in formulation, economic benefits and units of activity
PHYTEX 500 when added to feed at the recommended levels, allows for savings
of 1.5% protein and 0.1 % phosphorous in formulation. This represents a savings
of at least 600% its cost of inlusion per Mt of feed. One unit of activity of
phytase in PHYTEX 500 is the amount of enzyme required to liberate 1 micromole
of inorganic phosphorous per minute under the conditions of its assay method.
. One AGR U* (unit of activity) is the amount of enzyme required to produce,together,
an increase of 0.1% (w/w) of liberated phosphorous and an increase of 1.5 %
( w/w) soluble protein in a gram of standard formulated feed ration, at pH 5
, at 37ºC. PHYTEX contains 5000 AGR U* per gram.
Other benefits:
Inositol Hexaphosphate (IH) , binds minerals, such as iron, calcium, magnesium
in feed, diminishing their bioavailability. It also forms an undissolved complex
with protein which prevents activity of digestive enzymes, decreasing digestability
of proteins and their functions, such as emulsifying.Phosphoric acid in (IH)
can not be used by single stomach animals. Therefore, the need to add inororganic
phosphate in feeds as an additive. However, (IH) is secreted in feces, creating
environmental problems in large swine operations.
In the Netherlands, the use of poultry manure as a fertilizer is regulated
per regards to the amount of phosphate (P2O5) per acre.
Rate of application
100 grams per MT of feed. It can be pre-mixed or dispensed directly into the
mixer without pre-mixing. This inclusion rate provide 500,000 AGR U* units per
Mt of feed.
Charcteristics
Activity Vrs. Temperature: 100% Act.@ 60 ºC. ( Range 48ºC to 68ºC
) . Activity Vrs. pH : 100% Act. @ pH 6. (Range from 5.5 to 7). However, PHYTEX
500 can be pelletized at maximum: 80 º C for 30 seconds. max with an aproxímate
5 % to 10% l enzyme loss. It is 100 % soluble in water. For maximum effect,
add after pelleting.
Packaging
PHYTEX 500 is packed for bulk shipments in 20 kg boxes lined with special poly-
plastic bag.It can also be packed in smaller polybags as required by customer-
AMMINOGRAM INTERPRETATION
- The hydrolytic action of PHYTEX 500 on innositol hexaphosphate increases the
bio-availability of phosphorous, as well as other nutrients in feeds. The presence
of inositol hexaphosphate binds minerals and forms an undissolved complex with
proteins, diminishing the bioavailability of nutrients in feeds. The breaking
up of inositol hexaphosphate by PHYTEX 500, not only releases phosphorous ,
but a number of other nutrients , as expressed by the accompanying aminogram.
The values that appear in the amminogram are in terms of percentages and only
take into account the hydrolytic action of PHYTEX 500 . The percentages are
directly related to the PHYTEX 500 rate of inclusion in the feed ( grams / Mt
of feed).
In order to determine the % additional value of each nutrient made available
to the animal due to each enzyme's hydrolytic action , the following formula
should be used:
Thus the nutrional value for each nutrient in % per Mt of feed equals:
Nutrient's ( % ) value in the amminogram X ( Dosage in grams ) PHYTEX
500 / MT of Feed
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1,000,000 grams / TM
Making this calculation for each nutrient in the amminogram , will determine
the total savings in formulation by PHYTEX 500 .
METHOD OF ASSAY TO DETERMINE AGRI*U ( UNITS)
DEFINITIONS:
AGRI*U definition:
An AGRI*U (Unit) is the amount of PHYTEX 500 required to produce, together,
an additional 1.5% of soluble protein and an additional 0.1% free phosphorous
in feed rations.
One gram of PHYTEX contains 5,000 units of AGRI*U
MODE OF ACTION
The hydrolytic action of PHYTEX 500 on inositol hexaphosphate (IH) increases
the bioavailabilty of phophorous , as well as proteins in feed rations. Inositol
hexaphosphate (IH) binds minerals and undissolved complexes (that bind proteins),
diminishing the bioavailability of these proteins.in the animal gut. The breaking
up of (IH) by PHYTEX 500 releases free phosphorous and unbinds these protein,
making them more bioavailable to the animal. This action allows for formulating
rations with less phosphorous and less protein, producing a savings in said
formulation.
METHOD OF ASSAY
The object of the method of assay is to determine the increase of free phosphorous
and soluble protein in the ration in the ration.
1) Take one 2 kg of poultry grower feed and mix in two separate jars , each,
with a 1 liter solution ( Ph 5) simulating the gastric juice composition.
2) In one jar, apply 1 (one) gram of PHYTEX 500.
3) The other jar (control) , stays blank.
4) Incubate both jars for 24 hours, at gut temperature while stirring mildly
and continously .
5) After 24 hours analyze for free phosphorous by means of a spectrometer and
for soluble protein.
ENGOREPROD 24-37 20080706
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