Pig Industry - As transporting fresh semen - Genetics


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16/03/2006

Engormix.com
Argentina

As transporting fresh semen

does Somebody know if there is not problem if I transport fresh semen we say a time of 2 hours toward the dilution center in a water heater, will he/she Have repercución in the fertility of the sperms? or is it to forces that the semetales should be in the same place that the dilution center? It is that we don't have enough the space for both. Somebody that please has experience in the matter gives me their opinion, thank you compass.

17/03/2006

Rafael Villegas Oaks
Mexico

Nosostros was working some hogs, in a farm to the outskirts of Texcoco, Edo. Mex., and of there we took it 2 hrs. to a laboratory to evaluate it and to elaborate the doses, and to cool it up to 16°C, and we made the following thing:
to) after receiving the rich fraction of sperm, and having the care of straining, the secretions of the Gl. Bulbulouretrales tapioca Granules, we proceeded to incorporate in similar proportions the diluter to corporal temperature.
b) Of there the mixture placed it in a water heater of wide mouth, and we took it to the laboratory.
c) In the laboratory, he/she took a small sample for their evaluation, and with the rest of the mixture he/she was incorporated another point the same as the one ejaculated original.
d) Already with the results of the evaluation, you proceeded then to complete the rest of the diluter, all is enough for the dose total that you/they throw the calculations.

I comment you that so much the viability of the fresh semen as of the one diluted, they were very similar, obviously it was better the cool air, but when not having other, this was a viable alternative. The size of the litter didn't also vary, still with fresh semen.
What diluter are you using?

Luck.

17/03/2006

Thin José
Argentina

Friend Vega, I can tell him that my experience with pig semen without diluting for a term of one hour is good, I have not had problems of quality after having diluted neither of fertility. I have always taken care of the range of temperature, (37º, and never below 35º).
Equally I am very careful with the transport that doesn't suffer vibrations, neither blows or milk shakes, the ideal thing is trasportar the water heater container in the hands to avoid the abrupt movements of the vehicle.
As for the 2 hours of time, I believe that it would be necessary to prove, since the pig semen is the most rustic thing and aguantador that he/she has played me to elaborate, compared with the one of bovine, ovino, caprino, rabbit.
I wait it is he of utility my experience.
Cordial greetings.

Thin José

17/03/2006

PEDRO VELTRI
Argentina

you will Surely lose important part of sperms, but I don't believe that you will have problems, provided you don't have high temperatures.

17/03/2006

Jorge Perea Gayosso
Mexico

Friend:
For the transport of semen, you should remember that while more variations of temperature and more movements, the viability diminishes.
They have been to make many things in this sense, and the results are always irregular.
He/she tries to make the things well and to the first one, the fertility is the beginning of a good work program, you don't scant in resources, time, or spaces.
He/she thinks that if you had sires with it mounts direct, the cost would be incredibly but high, only with the value of the males, more the feeding, the substitutions the vaccines, the medications, etc.
If you will work a (CO.) Center of Artificial Insemination, you should make it from the beginning in the correct way. Good diluente, good quality of water, good males, good team, good material, etc.
Advise you with professionals as Minitube, or IMV, or Magapor, all have good systems, and excellent technicians that will help you to mount your CO., in the best way.
Greetings.

17/03/2006

Alonso Quirós
Costa Rica

My recommendation is to try to be able to maintain the innocuous semen, since the transport could be the most critical point in control, that which affects me directly the fertility of the sperms.
Greetings,

Alonso Quiros

18/03/2006

Jesus Antonio García Jiménez
Mexico

there is not any problem with the semen, provided the water heater has a temperature average of 35 C° so that they continue alive the sperms and have a good fertility, I recommend you that you put the sperms in a clean and sterilized recipient, as for example flasks, or rehearsal tubes, and put that flask in the water heater with water to a temperature of 35 C° during the lapse in that you arrive to the dilution center.
But before transporting the semen, you should know the techniques to milk the sire, I wait that you know, and if you don't know the technique I put on to your you order. It sends a message and I will be able to lean on with all pleasure.
Attn.

Engineer Jesus García Jimenez

18/03/2006

jose sacred Moorish ulloa
Mexico

I Worked four years in the process and shipment of semen, reason why I send you some advice that I wait they are you of utility.
Daily, the 365 days of the year, we prepared around 1200 á 1500 dose of semen. The important thing is the cleaning in the gathering and the dilution of the semen the quickest thing possible.
To prepare hieleras with coolant so that to the moment to keep the semen they are moderated and lower the semen the quickest thing possible to the 17 centigrade degrees. This has their logical explanation: if the semen is not gone down to that temperature, it continues with its normal motilidad and there is a lot of mortality of sperms, and studies have demonstrated that the most appropriate temperature for its transport and conservation is between the 17 and 20 centigrade degrees.
Later on the hieleras was sealed and they were sent from the municipality of Nochistlan Zac, until Acatic Jalisco, where they were most of the stomaches.
If I was not explicit and you have left some doubt, I am to your orders.

18/03/2006

Adriana Scaramuzza
Argentina

Dear Rosemary Vega:
I believe that the critical point that you have in the transport of fresh semen for a lapse of time of 2 hs is that, if you don't include diluter in the one ejaculated collected, you should be very strict with the temperatures of the water heater and of the semen. The same ones should be CONSTANT and UNIFORMS along the transport. The variations of temperature go against the viability of the sperms. He/she remembers that the semen should have a temperature among the 37ºC aprox., and in the water heater the same ones should be superior to the 38º-39º C, depending on the ambient temperature (you should sometimes take it at 40ºC, in strong winters).
The second important point to keep in mind, is the vibration to which will be subjected this sample. If it suffers a lot of agitation, don't hope to have a semen with many alive sperms. If they don't lose temper with agitations or soft vibrations.
As for the seminal fertility, it depends on respecting these two previously mentioned important points. The use of the diluter is so that the sperms obtain the necessary energy to conserve its mobility and don't spend that of their reservation, conserving the biggest quantity in alive sperms and with motilidad.
The most important thing is that the obtaining of the semen is correct (to filter the tapioca granules segregated by the Gland. Bulbouretrales, being these discarded), and to cover with an aluminum paper the recipient where the seminal content is, which should be previously well clean. During the whole time that will demand the transport, it will be protected of the sun and the powder.
I wait to have contributed you something to your restlessness. Any restlessness, don't doubt to consult me.
Until soon and luck.

Vet. Adriana Scaramuzza

18/03/2006

Guillermo Fernando González Necoechea
Mexico

you should not be afraid when transporting the semen, he/she only takes care that he/she doesn't become agitated too much in the transport, take care him of the solar light, the heat, humidity and extreme cold. I recommend you that you wrap the ampolletas in a cloth and you put them inside an unicel box or water heater like in those that the vaccines and other biological ones are transported.
I have come working this way the semen of my hogs for more than 2 years with excellent results, the ideal temperature oscillates between 17 and 22 centigrade degrees, it avoids to the maximum how you mecionaba that moves your deposit abruptly, and you won't have problem some.
I wait and they serve you my advice.
Attn.

L.C. AND M.V.Z. Guillermo Fernando González Necoechea
Distributor PURINA / Apizaco, Tlaxcala. Mexico

18/03/2006

Euclides Garavito Garzón
Colombia

Making a reading of the answers that you/they have contributed you, very valuable by the way, it assaults me a doubt: two data of temperature, in different comments, they are reported, one sustains that the best thing is to 35 centigrade degrees and another opinion speaks of 17 Degrees in hieleras. Which of two o'clock he/she will have better result?

19/03/2006

Carlos Alberto González Marthell
Mexico

To the friend Euclides does comment him that the temperature to which the semen is gathered should be approximately already of 37 °C, and the conservation temperature diluted the semen is of among 15 á 17 °C, do some manage 15°C, other 16, and in other occasions 17 °C. The important thing here is that the variations are not very abrupt, the error range should be of when a lot + - 1°C.

19/03/2006

Carlos Alberto González Marthell
Mexico

Romeo, I greet you and I comment you that the problems that you could have would be the variation of temperature in the transfer, but possibly this could reduce it with the use of an I fear of double bubble, in which inside him water is placed to the wanted temperature, and here I put it at 40°C so that in interior of the water heater he/she has 37°C, and I have made tests and he/she has stayed constant until for 4 Hrs. Another of the problems that you could have is the aglutinamiento, but this could reduce it with a tip that I will give you, to put 100 ml. of diluente (I am distributor of MR-TO and consequently it is the one that I occupy) moderated 17 °C (important the temperature) in the bottom of the bag of it gathers of the semen. This will help as shock and to avoid the aglutinamiento. I wait it works you, of equal it forms I share the friend's idea that recommends not to scant since of there it will be the success of the business, but if there is the readiness more than anything of resources, it is necessary to look for alternative. Perhaps you could condition a small space and without many steps, mainly of lava of instrumental, you would make it there same where you gather, with the use of polipapel bags I don't wash anything of the intrumental, only the mouthwashes of the bottles, and in few steps I make the preparation of the doses. I HAVE A DOUBT NOW WITH THE COMMENTS THAT I READ, OR PERHAPS I UNDERSTOOD BAD, WITH RESPECT IN THAT IT IS NECESSARY TO LOWER THE QUICK MA POSSIBLE THE TEMPERATURE TO THE SEMEN: AS IT WOULD BE MADE, AND IS MY DOUBT FOR THAT WHEN MAKING THIS, WOULD NOT THERE BE THERMAL CRASH? THANK YOU.

19/03/2006

lucio andrade medina
Venezuela

FRIEND: take into account the differences that he/she will have as for temperature for several reasons (diminishing viability and increasing esperm. dead), because he/she will have them!!! The teams not always work to the perfection, and the human errors are many unavoidable times. Another point to take into account is that to take the semen he/she makes it in hot 37 degrees, but to bring it he/she makes it in cold 17 degrees. My recommendation is that it doesn't save little money to bounce much more. Do avoid headaches that you for experience that will have them.

20/03/2006

MIGUEL ANGEL MARSERO
Argentina

My experience tells me that you could take it during those hours without beating it too much. You should also control the temperature that is not bigger than 38 degrees neither smaller than 35. I wait you are successful.

20/03/2006

Jorge Perea Gayosso
Mexico

Dear Friend:
Without minimizing the valuable comments of the other colleagues, I suggest you that you advise yourself of professional people of companies that are devoted to this as Minitube, IMV, or Magapor, all these systems are very good and they have very capable professionals.
Moving semen without diluting and without cooling, it is always a high risk, many have made it and they will follow it making, but it is not the best thing.
I have done good results in farms with a post in-situ, that is to say that the semen takes place there same, it is collected, it dilutes and it packs in the same farm, and it is applied fresh, and if it has more than enough, they cool it and they conserve between 15 and 17 degrees.
Don't give turns to the matter.
Well and to the first one it is always the best thing.
Greetings.

21/03/2006

Carlos Alberto González Marthell
Mexico

Friend Romeo, forgot me to comment you that I have qualified personnel that it can advise you. As well they mention it, I represent KUBUUS, we manage the one MR-TO diluter of long duration (7 days), without minimizing those that are recommending you, but we have also obtained good results in the farms that we have advised. There is even here an article that compares results of experiments made with the different diluters that exist. You could also consult them to enlarge your approach in this respect.

26/03/2006

walter guizado eagle
Peru

Hello, I am from Peru. The best thing is to make the dilution in the capable one the quickest thing possible because the water heater doesn't only offer the appropriate conditions, and obviously the quantity of sperm will shrink. I recommend you that you use microscope.

12/04/2006

Lumpish Sanchez Luis Servando
Mexico

you Maintain a good cleaning when gathering the semen, it uses a good diluter, maintain it under the 35 degrees, and it offers that he/she doesn't move too much in the transport. You will have this way minimum reductions, and when the circumstances allow it, it transfers the sire in a comfortable way...
Greetings.

06/07/2006

andres sandoval
Colombia

The HYGIENIC gathering (to avoid a later irreversible contamination), is it key, besides the data of temperature for the gathering and transport of the semen already mentioned.

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