Hello I am Antonio Mendez Lugo known as The Toñon in Mexico, I belonged to the pioneers in the implementation of Internal Banks of Semen in farms before sanitary problems as the PRRS, in that time it was a good solution, but now the aspect sanity is the but important, besides having a genetics, avant-garde, I am devoted to give service since to my clients I am distributor of several companies dedicated to the implementos production and teams, as for the collection temperature the semen is collected in general from 37 to 37.5°C. usually, we use water heaters of dry ice or poliestireno, previously some heated the water heaters, even some make it in some cameras with focuses, (observe many that were to but of 40°C. and to the moment to receive it the semen it was affected), in the personal thing I prefer the water heater that I mention you and not to heat it, the raw semen is conserved well and but time, chekamos motilidad masal, in drop to 10 and 40 increases and progressive with cubreobjetos in 40 increases, this is subjective, since it depends on the operative's experience and also that the portaobjetos is between 35 and 37 °c. then we evaluate the concentration for diverse methods, Camera of Neuvauer, of Burker or fotocolorimetro, and we carry out a tint Eosina Nigrosina for Morphology, since it is determined the I number of dose, he/she is carried out the isothermic dilusion, in general we use to extend of half duration for 4 days, we allow the doses to rest for one hour and later on they are introduced to a conservative of semen or incubator of low temperature to be conserved from 15 to 18 °C. there are of diverse types and marks and we have seen that this range is the appropriate one, they should mix slowly every 12 hours, either bottle, tube or bag, the truth that we don't use the bathroom maria to heat them before applying them, since excellent results have been had without making this technique recommended for European or Foreign, perhaps for their climates, also many times recommendations are made that our employees don't understand well, I believe that it should be been I practice and less obscurantista like they are usually some people that instead of simplifying the work routines, they complicate them but, in the personal thing I advise myself for People but capable that your servant when I have my doubts, fortunately here in mexico we have very capable and honest people, as for the integrity of the acrosoma, I believe that it is strange the presentation of a defect d genetic eorigen, some companies mentioned here that if you didn't have a Microscope of contrast of phases you could not have a high security in the evaluation, but that for my it is commercial roll, I believe that if you doubt of a Hog, there the Universities are, he/she receives a greeting and I am to their you order for any explanation
attn. MVZ. Antonio Mendez |
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